<ruby id="3p5pr"><table id="3p5pr"><ol id="3p5pr"></ol></table></ruby>
<dd id="3p5pr"></dd>
<button id="3p5pr"><tr id="3p5pr"></tr></button>
<em id="3p5pr"></em>
<button id="3p5pr"><acronym id="3p5pr"></acronym></button>

      <span id="3p5pr"></span>
      <dd id="3p5pr"><center id="3p5pr"></center></dd>
      <dd id="3p5pr"><noscript id="3p5pr"></noscript></dd>

        <tbody id="3p5pr"></tbody>
          <nav id="3p5pr"></nav>

            <th id="3p5pr"></th>
            <li id="3p5pr"></li>
            <th id="3p5pr"></th><rp id="3p5pr"></rp><em id="3p5pr"><tr id="3p5pr"><kbd id="3p5pr"></kbd></tr></em>
            <tbody id="3p5pr"><pre id="3p5pr"></pre></tbody>
            1. Pylon Technology

              A novel approach to immunoassays

              Immunoassays are ubiquitous in life science research, in clinical diagnostics and often a key element in clinical trials for biopharmaceuticals. Yet, current technology has not kept up with ever-changing needs.

              In research, scientists continue to look for higher sensitivity and better precision and to achieve them with a lower sample volume. To answer complex questions and accelerate discovery, they need an immunoassay platform with built-in flexibility for rapid assay development and ready adaption of assay protocols for changing analytical requirements. Ease of operation is critical, with shortage of trained personnel. And cost efficiency is key.

              In the clinical environment, the continuing push to improve healthcare efficiency is heightening the importance of near patient testing and faster time to results to support clinical decisions. Yet, it has proven challenging to deliver the level of assay performance that is more commonly associated with large central lab analyzers in the near patient environment with less infrastructure, lower operator skills and the need for faster time to results to fit into the clinical workflow.

              Pylon changes everything

              Pylon immunoassays incorporate advanced yet inherently simple concepts to deliver state-of-the-art performance without reliance on infrastructure often available only in the central laboratory. And by drawing upon proven fiber optics and semiconductor manufacturing knowhow, high-volume, low-cost implementation of the Pylon technology is achieved.

              Pylon Instrumentation

              Pylon test strip

              Innovative all-in-one, unitized packaging

              To achieve the multiple goals of advanced performance, ease of use, versatility in specimen requirements and scalability, Pylon immunoassay replaces conventional methodology with a Pylon test strip that contains everything needed to perform an immunoassay. The Pylon quartz-glass probe tip, coated with analyte-specific antibody, moves from well to well as it picks up the sample and goes through the assay steps of a classic sandwich assay. No fluidics are required, reducing instrument complexity and associated cost and maintenance.

              Watch Pylon test strip in action.

              View on on YouTube.com View on Youku.com

              Pylon cyclic amplification

              Redefining immunoassay performance

              Once the analyte in the sample binds with the antibody on the Pylon probe tip, the probe tip is dipped into the biotinylated antibody and then the Cy5-streptavidin-fluorescence (Cy5-SA) reagent. The probe tip is coated with a biocompatible polymer formulated to eliminate nonspecific binding. Signal amplification is achieved by embedding multiple copies of Cy5-SA in an inert polysaccharide matrix. The labeling cycle can be repeated to further enhance the signal.


              Achieving high sensitivity

              Pylon immunoassays combine the stability of fluorescence with polymer-enabled amplification to enhance signal while avoiding nonspecific binding and proximity quenching. The result is subpicogram per milliliter (pg/mL) detection of proteins in a short assay time of about 20 minutes.

              Inert polysaccharide matrix enhances sensitivity without increasing nonspecific binding

              Maintaining a low background is critical in achieving high sensitivity. As shown in the figure, the polysaccharide boosts the immune-specific signal without a significant increase in nonspecific binding.

              Watch Pylon cyclic amplification in action.

              View on YouTube.com View on Youku.com


              In Pylon assays, the high affinity of streptavidin and biotin is a key to precision, as is the relatively narrow size distribution of the high molecular weight biopolymer matrix in which the fluorescence label is embedded. The use of Cy5-streptavidin-polysaccharide polymer as a common reagent for all Pylon assays ensures lot-to-lot as well as assay-to-assay consistency. And the inherently more stable, dry reagent format enhances performance. For example, for the Pylon PCT Assay, the intra-assay and inter-assay CV is 7–10% in the clinically relevant PCT concentration range.

              Wide-range protocol

              For analytes that have an inherently broad concentration range (e.g., β-HCG), the Pylon assay protocol includes two sequences for each sample: one optimized for high concentrations (using short binding times and low flow rates), and the other optimized for low-end detection (using a longer binding time and higher flow rate). This avoids having to dilute the sample following an out-of-range assay run, and to repeat the analysis, saving the cost and time associated with a rerun. The Pylon β-HCG Assay has a detection range of 1 to 300,000 IU/L.

              韩国三级中文字幕hd 女人18毛片a级毛片| 国产在线精品一区二区不卡| 99在线精品国自产拍不卡| 黑人巨大40厘米重口无码| 琪琪网最新伦永久观看2019| 精品国产品国语在线不卡| 天天爽夜夜爽人人爽| 中文字幕人妻熟人妻熟丝袜美| 无限在线观看完整版免费视频| 国产乱了伦视频大全亚琴影院| gogo国模啪啪人体|